Description

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. Their main function is the removal of cellular debris and invading pathogens [1]. Hepatic macrophages, which are also known as Kupffer cells, reside within the lumen of liver sinusoids. Hepatic macrophages protect the liver by responding to pathogens and metastatic cells, whilst tolerating harmless self and foreign antigens, which enter via blood flow through portal vein and hepatic artery [2]. Recent studies have shown that hepatic macrophages play an important role in fibrosis, liver inflammation, alcoholic liver disease, fatty liver disease, liver transplantation and more [3-6]. Macrophages isolated from fresh rat liver tissue serve as an excellent model for studying their functions under normal physiological and pathological conditions. Macrophages can be identified by specific expression of a number of proteins including F4/80 (mice)/EMR1 (human), CD14, CD11b, MAC-1/MAC-3 and CD68 by flow cytometry or immunocytochemical staining [7].

RHMa from ScienCell Research Laboratories are isolated from neonatal day 2 rat liver tissue. Cells are cryopreserved after purification and delivered frozen. Each vial contains > 1 x 10^6 cells in 1 ml volume. RHMa are characterized by immunofluorescence with antibody to F4/80. RHMa are negative for mycoplasma, bacteria, yeast and fungi. RHMa is guaranteed to further culture in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. No. 1921) for the culturing of RHMa in vitro.

Product Use

RHMa are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1]. Wynn TA and Barron L. (2010). Semin Liver Dis. 30:245-57.
[2]. Liaskou E, Wilson DV and Oo YH. (2012). Mediators Inflamm. 2012: 949157.
[3]. Ajakaiye M, Jacob A, Wu R, Zhou M, et al. (2011). Biochem Biophys Res Commun. 409: 406–411.
[4]. Bieghs V, Verheyen F, van Gorp PJ, Hendrikx T, et al. (2012) PLoS One. 7: e34378.
[5]. Tian Y, Jochum W, Georgiev P, Moritz W, et al. (2006). Proc Natl Acad Sci U S A. 103: 4598–4603.
[6]. Seki E, De Minicis S, Inokuchi S, Taura K, et al. (2009). Hepatology. 50: 185–197.
[7]. Gordon S and Taylor P. (2005). Nature Reviews Immunology. 5:953-964.