上海中乔新舟生物科技有限公司是美国Sciencell公司官网公布的一级代理
       
       美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。

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产品：人食管成纤维细胞

货号：2730


Description

Esophageal fibroblasts which line the lamina propria of the upper andlower esophagus are mesenchymal cells that originated from the embryonicmesoderm. They have been shown to play an important role in angiogenesisthrough TGF-β and VEGF signaling pathways in the highly angiogenicesophageal squamous cell carcinoma [1]. Predominantly, fibroblastssecrete a nonrigid extracellular matrix that is both rich in type Iand/or type III collagen [2]. They are responsible for much of thesynthesis of extracellular matrix in the connective tissues and playmajor roles in wound healing. Because they are one of the simplest typesof cells to grow in culture fibroblasts have been extensively used for awide range of cellular and molecular studies [3]. In addition,fibroblasts’ durability makes them amenable to a wide variety ofmanipulations ranging from gene transfection to microinjection.

The HEF cells from ScienCell Research Laboratories are isolated fromhuman esophageal tissue. HEF are cryopreserved at passage one anddelivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume.HEF are characterized by immunofluorescence with antibody specific tofibronectin. HEF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria,yeast and fungi. HEF are guaranteed to further expand for 15 populationdoublings at the conditions provided by ScienCell Research Laboratories.


Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for theculturing of HEF in vitro


Product Use

HEF are for research use only. It is not approved for human or animaluse, or for application in in vitro diagnostic procedures.


Storage

Directly and immediately transfer cells from dry ice to liquid nitrogenupon receiving and keep the cells in liquid nitrogen until cell cultureis needed for experiments.


Shipping

Dry ice.


Reference

[1] Noma, K., Smalley, K., Lioni, M., Naomoto, Y., et al. (2008).Gastroenterology. 134:1981-93.
[2] Gabbiani, G., Rungger-Brandle, E., et al.(1981). The Fibroblast. .1-50. Handbook of Inflammation, Vol. 3.
[3] Conrad, W., Hart, W., Chen, Y. (1977). J. Cell Sci. 26:119-37.