Opti-MEM® I Reduced Serum Medium-null-资讯-生物在线

Opti-MEM® I Reduced Serum Medium

作者:北京索莱宝科技有限公司 2016-05-11T00:00 (访问量:5241)

 

 

Opti-MEM® I Reduced Serum Medium

 

基本信息

产品名称:Opti-MEM I Reduced Serum Medium

货号:31985070

品牌:Gibgo

规格:500ml

储存条件:2-8

用途:细胞培养

描述

Opti-MEM® Reduced-Serum Medium is an improved MinimalEssential Medium (MEM) that allows for a reduction of FetalBovine Serumsupplementation by at least 50% with no change to growth rateor morphology. Opti-MEM® is also recommended for use with cationic lipidtransfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be usedwith a variety of suspension and adherent mammalian cells, including Sp2, AE-1,CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a varietyof Gibco® Opti-MEM® modificationsfor a range of cell culture applications.

This Opti-MEM® is modified as follows:

With

• L-glutamine

• Phenol Red

The complete formulation is confidential. For moreinformation, please contact TechnicalServices.

Gibco® Opti-MEM® is a unique media that contains insulin,transferrin, hypoxanthine, thymidine, and trace elements. These additionalcomponents allow for a reduction in serum supplementation by at least 50%.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animaltherapeutic use. Uses other than the intended use may be a violation of locallaw.

cGMP Manufacturing and Quality System
Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in GrandIsland, New York. The facility is registered with the FDA as a medical devicemanufacturer and is certified to ISO 13485 and ISO 9001 standards. For supplychain continuity, Life Technologiesoffers an identical Gibco® Opti-MEM® productmade in our Scotland facility (31985-047). This facility is registered with theFDA as a medical device manufacturer and is certified to the ISO 13485standard.

Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g / L) andtherefore requires a 5-10% CO2 environment to maintain physiologicalpH.
     

常见问题解答

   My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinizefor a shorter time or use less trypsin. Mycoplasma contamination could alsocause this problem. Segregate your culture and test for mycoplasma infection.Lastly, check for attachment factors in the medium.

 How much serum is in Opti-MEM™ I Medium?

None. The term “reduced serum” means that one can reducethe amount of serum they use with classical cell culture media. When workingwith Opti-MEM™ I medium, most cells that are routinely cultured inserum-supplemented medium may be transferred directly into Opti-MEM™ I mediumwith a minimum of 50% reduction in serum.

 

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